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KMID : 0359319920320020195
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Korean Journal of Veterinary Research
1992 Volume.32 No. 2 p.195 ~ p.205
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Comparison of soluble antigens from Leptospira interrogans serovars by SDS - PAGE , Crossed Immunoelectrophoresis and Immunoblotting
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Abstract
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The soluble antigen profiles and antigenic specificities of Leptospira interrogans serovars icterhaemorrhagiae, canicola, pomona and hardjo were examined by SDS-polyacrylamide gel electrophoresis, crossed immunoeletrophoresis and immunoblotting.
The profiles of protein, glycoprotein and fraction containing N-acetylglucosamine of 4 serovars were compared. The protein profiles of 4 serovars were very similar except the range of 14,400 to 30,000 daltons. Molecular weight of glycoprotein of 1, pomona was lower than other serovars. L canicola showed extra N-acetylglucosamine bands having molecular weight of 82,000 and 90,000 daltons.
In crossed immunoelectrophoresis, a close antigenic relationship was found between L icterohaemorrhagiae and L canicola.
In immunoblottings conducted with soluble antigens and rabbit antisera of 4 serovars, Leptospira interrogans serovars possessed cross-reactive antigens and serovar-specific antigens. The molecular weights of serovar-specific antigens were 45,000, 82,000 and 90,000, 31,000 and 24,000 daltons in L icterohaemorrhagiae, L canicola, L pomona, and L hardjo, respectively.
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